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Fig. 5. Grem1, but not SHH, rescues Fgf8 and Bmp2
expression in the AER of Gre
ORF/ORF limb
buds. All grafted limb buds are forelimb buds (E10.5) of Grem1 mutant
embryos. Limb buds either received Shh (red arrow) or Grem1
(green arrow)-expressing cell aggregates and were cultured for 15 hours prior
to analysis. White arrowheads indicate the endogenous expression domains; blue
arrowheads and asterisks indicate the induced expression. (A) Bmp2
expression in a non-grafted control limb bud of a mutant embryo. (B) Posterior
grafts of Shh-expressing cell aggregates fail to rescue Bmp2
expression. (C,D) Posterior grafts of Grem1-expressing cells induce
Bmp2 expression in the AER (D), while no Bmp2 transcripts
are detected in the AER of non-grafted mutant limb buds (C). Note also the
enhancement of mesenchymal Bmp2 expression (D). (E,F) Posterior
grafts of Shh-expressing cells do not rescue Fgf8 expression
in the AER (F) in comparison with a non-grafted mutant limb buds (E). (G,H)
Posterior grafts of Grem1-expressing cells induce upregulation of
Fgf8 expression in the AER (H) in comparison with endogenous
Fgf8 expression in non-grafted mutant limb buds (G). (A-D) Dorsal
views with posterior towards the bottom and distal towards the right; (E-H)
posterior is towards the bottom and dorsal towards the left.
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