DEV01229 Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Unc-GFP does not exchange between centrioles and cytoplasm. Panels show centrioles in spermatocytes cultured from UNC-GFP-expressing adult testis, before and after photobleaching. GFP was bleached with a confocal laser, focused on one of a pair of centrioles (arrows); pairs were imaged before bleaching and at 1-2 minute intervals afterwards. Most unbleached centrioles retained a constant level of fluorescence over this time, but no fluorescence recovery was seen on the bleached centriole. Experiments were performed on cells 3-5 hours after cell dissection; cells retained normal morphology for 24-48 hours afterwards.  Eight experiments were performed, all with similar results.

• Supplemental Figure 2 -

  Fig. S2. Ectopically expressed Unc-GFP does not localize to centrioles. Confocal images of UNC-GFP (green) expressed in a blastoderm embryo (A,B) and in post-mitotic larval cells (C, salivary glands; D, tracheal cells; E, epidermal cells). Nuclei in A are labeled with propidium iodide (purple). UNC-GFP does not localize to centrioles or interfere with mitosis or cellularization in embryos. In postmitotic larval cells, extensive filamentous structures are labeled by ectopically expressed UNC-GFP. Scale bars: 5 mm.