|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Spermatogenesis defects in unc mutants. (A,B) Cysts of elongating
spermatids in wild-type (A) and unc mutant (B) testis, stained for
nuclei (red) and ß-tubulin (blue). Nuclei are clustered at the head of
wild-type cysts but are dispersed in unc mutants. (C) Nuclei in cysts
of an unc mutant testis, stained for DNA (red) and 
-tubulin
(blue). Two stages of nuclear condensation are present. Earlier,
less-condensed nuclei (left side of panel) are still associated with the
-tubulin-containing centriole adjuncts as in wild type, but mutant
nuclei (arrows) and adjuncts (arrowheads) frequently detach at later stages.
Mutant nuclei do not condense further than the stage seen in cross-section on
the right. (D-F) Ultrathin sections of wild-type (D) and mutant (E,F) testes.
Wild-type spermatids have a canonical 9+2 axoneme, associated with an
electron-dense mitochondrial derivative. Most mutant axonemes are partially or
completely disrupted, and lose their tight association with mitochondrial
derivatives. Scale bars: in A-C, 5 µm; in D,F, 0.2 µm.
|
