|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. unc encodes a large coiled-coil protein. (A) Complementation and
physical maps of the unc region at the base of the X chromosome.
Flies heterozygous for the overlapping deficiencies Df(1)S54 and
Df(1)B57 show an unc phenotype. The left breakpoint of
Df(1)S54 and breakpoints associated with three x-ray-induced
unc alleles are indicated. (B) The unc transcription unit,
with promoter fusion, rescuing transgene and GFP fusion constructs below. The
open reading frame of a cDNA clone (LP08350) was extended by 5'-RACE to
a 5' UTR with in-frame stop codons. A transgene including 2 kb of
upstream DNA fused to the balance of the cDNA restored normal sound-evoked
responses (C) to unc mutants. The same upstream DNA fragment was
fused to EGFP to report transcription from the unc promoter. To
examine protein localization, DNA encoding EGFP was appended to the
unc-coding sequence. (D) Predicted unc proteins from D.
melanogaster (D.m.), D. pseudoobscura (D.p.) and the mosquito
Anopheles gambiae (A.g.). Each protein includes several short
coiled-coil segments, separated by proline residues or proline-rich regions,
and a Lissencephaly 1 homology (LisH) motif (green box). The positions of stop
codons in five EMS-induced unc alleles are indicated in the
melanogaster protein. (E) Alignment of the LisH motifs from the
proteins in D and from the human ODF1 protein (H.s.).
|
