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Fig. 5. UNC-GFP localization in sensory neurons. (A,B) A singly innervated macrochaete bristle showing a focus of Unc-GFP labeling (arrow) near the end of the sensory process. The neuronal process and the ciliary outer segment are labeled with anti-HRP (red); autofluoresence from the cuticle outlines the bristle and socket in green. (C) Clustered spots of Unc-GFP (green) label the multiply innervated olfactory sensilla on the third antennal segment. Nuclei are labeled with propidium iodide (red). The boxed region is enlarged in the inset, without the nuclear labeling. (D) Two embryonic lateral chordotonal clusters. Anti-22C10 (red) labels the neuronal and inner dendritic segments, but not the ciliary outer segments. Each neuron shows two labeled spots (green, arrows), with the more proximal at the end of the inner segment. Scale bars: in A, 1 µm for A,B; in C, 20 µm for C, 4 µm for D.





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