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Fig. 8. Altered {gamma}-tubulin localization in unc mutant spermatocytes. (A) Unc-GFP (green) and anti-{gamma}-tubulin (blue) labeling cysts at multiple stages of development in a wild-type testis. DNA is labeled with propidium iodide (red). Boxes, enlarged below, include cells entering meiosis I (1) and exiting meiosis II (2). (B,C) {gamma}-Tubulin labeling in wild-type (B)and unc mutant (C) spermatocytes. In mutants, {gamma}-tubulin staining is reduced to small dots, each probably equivalent to a pair of centrioles in wild type. Scale bars: 10 µm.





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