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Fig. 3. (A) Numbers of neurospheres from E10.5 rostral telencephalic vesicles of
wild type (WT) or tenascin C (TNC) null mice (K/O) grown for seven days in the
presence of either fibroblast growth factor (FGF2) or epidermal growth factor
(EGF) (20 ng/ml). Note the lack of EGF-responsive neural stem cells in TNC
null cells as demonstrated by the absence of neurosphere formation in EGF.
Results represent mean±s.e.m. of three independent experiments
(Student's t-test: *P<0.001). (B) E10.5
neurospheres were grown for 5 days from heterozygous (+/–) or TNC null
(–/–) cells in the presence of FGF2 (20 ng/ml) and 0 or 10
µg/ml TNC as indicated. The Figure shows 4 gel lanes from the same Western
blot analysis of neurosphere proteins using antibodies against the EGFR, with
a long exposure required to visualise the low levels of EGFR protein at this
developmental stage. Note that EGFR is detected in heterozygous cells either
with or without added TNC (lanes 1-2). By contrast, FGF2 alone was not
sufficient to induce EGFR expression in the null cells after 5 days (lane 3),
and that EGFR expression was rescued by the addition of exogenous TNC for the
duration of the experiment (lane 4).
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