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Fig. 5. Expression of EGF receptor in neurospheres. Sections of mouse neurospheres grown from P0 pups in EGF (A) or FGF2 (B). There is a greater expression of EGF receptor in the neurospheres grown in FGF2 (compare A and B). This is confirmed by western blotting using an anti-EGF receptor antibody, as shown in C. Note also the increased expression of ß1 in spheres grown in EGF (lane E) when compared with spheres grown in FGF2 (lane F). Scale bar: 40 µm for A,B.





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