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Fig. 4. Increase of acinar cell apoptosis and ductal proliferation in orpk
mice after birth (P4). TUNEL staining showed an increase of apoptotic cells in
pancreas of orpk mice (B) when compared with wild-type mice (A).
Inset in B shows that no apoptotic cells could be detected in islets
(outlined) in orpk mice. Proliferation is increased in dilated ducts
of orpk mice, as shown by Ki-67 staining (C,D). Note that the number
of proliferating cells in orpk mice in dilated ducts (outlined in
white) is higher than in non-dilated ducts (outlined in yellow).
Quantification of apoptotic cells, measured as number of cells positive for
TUNEL staining per field of view (E; blue, wild type, n=5; red
orpk, n=5), showed an increase of cell apoptosis in orpk
mice. Proliferation indexes of pancreatic cells are given as number of
Ki-67-positive cells per mucin-positive, insulin-positive or amylase-positive
cells (F; wild type, n=5; orpk, n=5). Wild-type numbers for
apoptosis and proliferation were set to `1' to facilitate comparison (E,F).
Errors bars are shown as ±s.e.m.
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