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Fig. 5. Absence of pancreatic cilia and increased polycystin-2 expression in
orpk mice. In wild-type pancreatic sections (A) polycystin-2
expression is localized in intercalated ducts. Cilia were visualized using an
antibody against acetylated tubulin. Cilia are confined to the lumen of
intracinar and intercalated ducts (arrows). Acini (a) and intercalated ducts
(id) are outlined in yellow and white, respectively, for better visualization.
Inset shows Hematoxylin/Eosin staining of acini in wild type. A dramatic
decrease in cilia number is detected in orpk mice (B; arrow).
Polycystin-2 expression in orpk mice is increased and mislocalized in
intracinar dilated ducts. An acini with a severe intracinar ductal dilation is
outlined in yellow. Dilated intracinar ducts (outlined in red), a structure
that is not found in wild-type pancreas, show high polycystin-2 expression,
comparable to levels that are only observed in intercalated ducts of control
mice. Inset shows Hematoxylin/Eosin staining of a dilated intracinar duct in
orpk mice. Cilia are also present in islets in wild-type mice (C),
but are almost absent in orpk mice (D; arrow). Cilia are present at
embryonic stages (E15.5) in wild type (E) but very few could be detected in
orpk mice (F). Cilia length, shown in µm, is reduced in
orpk mice (G). Tissue sections were derived from three different
animals at each stage. Errors bars are shown as ±s.d. a, acini; id,
intercalated duct; dd, dilated ducts.
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