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Fig. 6. Differential regulation of progesterone receptor isoform by MTA1. (A) RT-PCR analysis of PR isoforms and K18 expression in 12-week-old virgin MTA1-TG and wild-type female mice. (B) Western blot analysis of PR isoforms in 12-week-old virgin MTA1-TG (line 31 and line 33) and wild-type female mice. (C) IHC detection of PR total and PRA isoform in mammary glands of wild-type and MTA1-TG mice. (D) Quantification of PR staining. Western blot analysis of T7-MTA1 expression in MCF-7/MTA1 (E) and HC11/MTA1 (F), and control clones using an anti-T7 monoclonal antibody. Western blot analysis of PR isoforms expression in MCF-7/MTA1 (G) and HC11/MTA1 (H).





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