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Fig. 8. Involution is severely affected in the mutant mammary glands. (A)
Hematoxylin and Eosin staining of mammary glands from wild-type mice (a,c,g)
and mammary glands from MTA1-TG mice (b,d,h). Day 1 of involution (a,b), day 3
of involution (c,d), day 21 of involution (g,h). Carmine Red-stained whole
mounts of mammary glands at day 21 of involution (e,f). Hyperplastic nodules
are present in MTA1-TG mice. (B) TUNEL analysis of wild-type (a,c) and MTA1-TG
(b,d) involuting mammary gland 1 day (a,b) and 3 days (c,d) of involution. (C)
Quantification of tunnel-positive cells. Ten 200x magnification fields
of view were randomly counted. The apoptosis index was calculated as follows:
(number of TUNEL positive cells/total number of cells)x100. Statistical
analysis was performed using Student's t-test. Error bars represent
s.e.m. (D) Western blot analysis of Bcl-XL expression in wild-type
and MTA1 transgenic mice at days 1, 3 and 7 of involution (Inv). Vinculin was
used as a loading control.
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