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Fig. 7. Cell proliferation and marker gene expression in the cardiac OFT. Cell proliferation was analyzed in transverse sections of controls (A) and Alk2/Wnt1-Cre mutants (B) by using BrdU incorporation assay. The mesenchyme of OFT cushions (arrows) is significantly reduced in size in Alk2 mutants (B) when compared with the corresponding tissues in controls (A). (A',B') High-magnification views of the OFT cushions indicated with arrows in A and B. Comparison of the proliferation indices for OFT cushion cells indicated that cell proliferation did not significantly differ between these two genotypes (n=3). In Alk2/Wnt1-Cre mutants, the level of Plxna2 (arrows, marker for NCCs) expression is diminished at E11.0 (D) compared with controls (C). Msx1, an effector of BMP signaling, is expressed in the proximal OFT mesenchyme (arrows) both in controls (E) and ALK/WNT1-Cre mutants (F). However, only the control samples display Msx1 expression in the distal OFT mesenchyme (arrowhead in E). Scale bars: in A, 200 µm for A,B; in A', 100 µm for A',B'; in C, 200 µm for C-F.

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