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Fig. 1. CUL-2 complex components and ZYG-11 are required for progression into meiotic anaphase II. (A) Histone H2B::GFP epifluorescence movie sequences of live zygotes of the indicated genotype. Time post-fertilization is indicated in the upper left corner (hours:minutes). Interphase denotes the initiation of chromosome decondensation after meiotic exit. The metaphase images are generally transverse views to show the pentagonal array except for the elc-1 (00:01) and zyg-11(mn40) (00:25), which are side views showing paired homologous chromosomes and sister chromatids, respectively. Arrowheads denote polar bodies. (B) The duration of meiosis I post-fertilization (left) and meiosis II (right) derived from histone H2B::GFP movies. See Materials and methods for the number of embryos analyzed. (C) Histone H2B::GFP images of separated metaphase II chromosomes in cul-2(RNAi) and cul-2(ek1) embryos. Scale bars: 5 µm.





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