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Fig. 2. The cul-2 meiotic phenotypes are not caused by a failure to remove the cohesin REC-8 from sister chromatids. (A) Immunofluorescence images of REC-8 in wild-type and cul-2(RNAi) embryos. Images of wild-type (top) and cul-2(RNAi) zygotes (bottom) stained with DAPI and anti-REC-8 antibody at the indicated meiotic stages. Note that in late metaphase II cul-2(RNAi) zygotes, REC-8 is not detected but chromosomes fail to segregate. Arrowheads indicate polar bodies. (B) Depletion of rec-8 by RNAi does not rescue the meiotic delay in cul-2(RNAi) zygotes. Histone H2B::GFP fluorescence movie sequences in rec-8(RNAi) or cul-2(RNAi); rec-8(RNAi) double RNAi embryos. In the rec-8(RNAi) zygote, chromosomes compress and rapidly move to the cell cortex between time points 00:17 and 00:22. Time from fertilization (hours:minutes) is shown in the upper left corner of images. Stars denote meiotic chromosomes or interphase pronuclei. Dashed lines denote the egg boundary; arrowheads denote polar bodies. Scale bars: 5 µm.

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