DEV01244 Supplemental Data

Files in this Data Supplement:

• Supplemental Table (PDF) - Table S1. Analysis of fixed wild-type, zyg-11(RNAi), cul-2(RNAi) and cyb-3(RNAi) embryos
• Movie 1 -

  Movie 1. In utero recording of a wild-type embryo expressing GFP-HIS, from fertilization until the two-cell stage. Note that embryos move through the spermatheca at fertilization. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 2 -

  Movie 2. Ex utero recording of a wild-type embryo expressing GFP-HIS, from metaphase of meiosis I until the two-cell stage. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 3 -

  Movie 3. In utero recording of a zyg-11(RNAi) embryo expressing GFP-HIS, from fertilization until the first S phase. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 4 -

  Movie 4. Ex utero recording of a cyb-3(RNAi) embryo expressing GFP-HIS, from metaphase of meiosis I until the first S phase. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 5 -

  Movie 5. Ex utero recording of a cul-2(RNAi) embryo expressing GFP-HIS, from metaphase of meiosis I until the first S phase. Note that this is a different embryo from the one shown in Fig. 1. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 6 -

  Movie 6. Ex utero recording of a zyg-11(mn40) cul-2(RNAi) embryo expressing GFP-HIS, from metaphase of meiosis I until the first S phase. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 7 -

  Movie 7. Ex utero recording of a zyg-11(mn40) cyb-3(RNAi) embryo expressing GFP-HIS, from metaphase of meiosis I until the first S phase. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 8 -

  Movie 8. Ex utero recording of a zyg-11(RNAi) embryo expressing GFP-HIS, GFP-TUB and GFP-PAR-2, from meiosis II until the two-cell stage. Note that the division is asymmetric as in wild type. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 9 -

  Movie 9. Ex utero recording of a zyg-11(RNAi) embryo expressing GFP-HIS, GFP-TUB and GFP-PAR-2, from meiosis II until the two-cell stage. Note that the division is asymmetric but inverted compared with wild type. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.

• Movie 10 -

  Movie 10. Ex utero recording of a zyg-11(RNAi) embryo expressing GFP-HIS, GFP-TUB and GFP-PAR-2, from meiosis II until the two-cell stage. Note that the division is symmetric and that the two resulting blastomeres divide as in par-2 mutant embryos. Embryos were recorded by dual DIC and GFP time-lapse video microscopy. Images were captured at 30-second time intervals. Display rate is 10 frames per second.