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Fig. 1. zyg-11, cul-2 and cyb-3 are required for progression
through meiosis II. (A) Images from time-lapse sequences of embryos expressing
GFP-HIS (see also Movies 1-7 at
http://dev.biologists.org/supplemental/).
Metaphase views are: polar (P), the spindle is viewed from the pole and five
homologues (meiosis I) or sister chromatids (meiosis II) are observed in a
circle around the sixth; lateral (L), the spindle is viewed from the side and
up to three pairs of homologues (meiosis I) or sister chromatids (meiosis II)
are observed; or intermediate (I), between the polar and lateral views. In
this and other figures, embryos are oriented with polar bodies to the left and
all panels of a kind are at the same magnification. Scale bar: 5 µm. (B)
Timing of events in embryos from fertilization (t=0) until the end of meiosis
II. Average duration of each stage, defined in
Table 1, is indicated. Letters
in the time-lines indicate the time at which the corresponding images in (A)
are taken. Vertical white lines indicate the time at which GFP-PAR-2 first
becomes enriched at the cell cortex in the vicinity of oocyte-derived
chromosomes in zyg-11(RNAi) (n=4) or
zyg-11(mn40) (n=3) embryos imaged starting at the
end of meiosis I.
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