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Fig. 2. zyg-11 is required for timely metaphase to anaphase transition and
M phase exit at meiosis II. (A-N) Lateral views of wild-type or
zyg-11(RNAi) embryos at the indicated stages, stained with
antibodies against 
-tubulin (A-C,G-J), or -tubulin and
phosphorylated histone H3 (D-F,K-N). Insets below panels A-C and G-J, as well
as the entire panels D-F and K-N are magnified views of a meiotic spindle and
have a width of 
7 µm. Insets below panels A-C and G-J show
-tubulin (green) and DNA (blue) on the left, and DNA alone on the
right. Panels D-F and K-N show -tubulin (green), phosphorylated histone
H3 (red) and DNA (blue) on the left, and phosphorylated histone H3 alone on
the right. To view polar bodies and sperm chromosomes, the DNA signal in the
low magnification images is a projection of several 1-µm confocal optical
sections. Arrowheads point to the first polar body, arrows indicate condensed
sperm DNA. Scale bar: 10 µm. Note that the focus of phosphorylated H3 lies
between homologues at metaphase I and sister chromatids at metaphase II in
both wild-type and zyg-11(RNAi) embryos. (O,P) Wild-type (O)
and zyg-11(RNAi) (P) anaphase II embryos expressing
GFP-CYB-3 stained with antibodies against -tubulin (not shown) and GFP
(red); DNA is shown in blue. (Q) Quantification of signal intensities in
wild-type (n=6), zyg-11(RNAi) (n=14) and
cul-2(RNAi) (n=19) embryos in metaphase II or
anaphase II stained as in (O); embryos were staged using the
anti--tubulin and DNA signals. The difference between wild type and
zyg-11(RNAi) or cul-2(RNAi) is
statistically significant (Student's t-test:
P=6x10-5 and P=4x10-6,
respectively).
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