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Fig. 3. cul-2 destabilizes GFP-ZYG-11; zyg-11 does not regulate GFP-ZIF-1. Images of paralyzed animals (A-C,H-J) or of fixed embryos stained with anti-GFP antibodies (D-F,K-M) from strains expressing GFP-ZYG-11 (A-F) or GFPZIF-1 (H-M). Plain outlines indicate oocytes, stippled outlines embryos. Note also the gut autofluorescence outside of embryos in live specimens. Scale bars: 50 µm. (G,N) Quantification of GFPZYG-11 (G) and GFP-ZIF-1 (N) signal intensities in fixed early embryos (i.e. <10 cell stage) of the indicated genotypes stained as in (D-F,K-M). Increase of GFP-ZYG-11 in cul-2(RNAi) is 16.4±3.0-fold, of GFP-ZYG-11 in elc-1(RNAi) is 17.3±3.2-fold, and of GFPZIF-1 in cul-2(RNAi) is 5.8±3.3-fold.

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