|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Inverted AP polarity during meiosis II delay in the absence of
zyg-11 or cul-2 function. Wild-type (A-D),
zyg-11(RNAi) (E-H), cul-2(RNAi) (I-L), and
cyb-3(RNAi) (M-P) embryos during meiosis II, and wild-type
embryos during the first mitotic cell cycle (Q-T), stained with antibodies
against 
-tubulin (green in merged image) and a polarity marker as
indicated (PAR-1, PAR-2, PAR-3 or PAR-1+PGL-1 to detect P granules; red in
merged image and viewed alone in the top panel); DNA is shown in blue in
merged image. Scale bar: 10 µm. Note that enrichment of PAR proteins and P
granules in the absence of zyg-11 or cul-2 function is
somewhat variable during meiosis II, and is less pronounced, both in intensity
and in lateral extent, than in wild-type embryos during the first mitotic cell
cycle.
|
