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Fig. 7. Evolution of polarity in zyg-11(RNAi) embryos. (A-C) Images from time-lapse sequences of zyg-11(RNAi) embryos expressing GFPPAR-2 and GFP-HIS, as well as GFP-TUB, shown during the meoisis II delay (left panel), the first mitotic division (middle panel) and the twoto four-cell stage transition (right panel) (see also movies 8-10 at http://dev.biologists.org/supplemental/). Recordings were started during the meiosis II delay. Arrowheads point to first polar body, arrows indicate condensed sperm DNA; stippled arrowhead and arrows indicate that these positions can be determined only from the movies. Scale bar: 10 µm. (D) Schematic representation of two-cell stage embryos (n=19) corresponding to recordings A (6 embryos), B (10 embryos) or C (3 embryos). See text for details.





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