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Fig. 5. The armadillo domain mediates neurogenesis promoted by ß-catenin and
RA acts synergistically to enhance ß-catenin-mediated neurogenesis. Cells
were seeded at high (106 cells/ml, HD) or low densities
(105 cells/ml, LD) and treated with or without RA (cells in panels
C,G,K,O were grown in KOSR media, see Materials and methods). Cells were fixed
and stained 3-4 days post-plating with ß-tubulin 3 (green) and
counterstained with Hoechst dye (blue). (A-D) Cells transfected with empty
vector. (E-H) Cells transfected with ß-catenin 
Armadillo. (I-L)
Cells transfected with ß-catenin C. (M-P) Cells transfected with
ß-catenin N. The armadillo domain of ß-catenin, but not the C
terminus, was required to overcome density-dependent inhibition of
neurogenesis.
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