Supplemental Figure (PDF) -
Fig.
S1. ICV injections
of beads coated with U0126 provide a prolonged blockade of pERK in the dLGN and
retina. (A) 24 hours after injection, pERK expression in the dLGN of treated
rats (P6, n=6)
is decreased with respect to control animals injected with beads-vehicle (B, n=3). (C,D) At 48 hours after
injection, pERK staining of treated rats (C, P4, n=5) is recovering to levels similar
to those of controls (D, n=5). (E, left) Immunoblots for pERK in the retina 24 hours after ICV
bilateral injections of beads-U0126 or beads-vehicle (C, control; T,
U0126-treated). (E, right) pERK levels assessed by immunoblot show a 69±17%
reduction in treated animals with respect to control animals (Student’s t-test, P<0.05). Each column is an average
of five different samples. Each sample pools together two retinas. Error bars
depict the s.d. Scale bar: 100 mm for A-D.
