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Fig. 3. Specific blockade of pERK in LGN neurons. (A) All pERK-positive cells (red
labeling) in the dLGN of P5 rats (n=5) co-localize with dLGN
projection neurons labeled with the retrograde tracer FluoroGold (green
labeling). (B) Schematic representation of visual cortex injections of
fluorescent latex beads in newborn rats. (C) A large number of retrogradely
labeled neurons are evident in the dLGN after injections of red fluorescent
latex beads in the primary visual cortex (n=4). (D) The red beads are
present throughout the cytoplasm of geniculo-cortical neurons labeled by
Fluorogold (n=3). (E) Cortical injections of beads-U0126 produce a
prolonged blockade of ERK activation in the dLGN ipsilateral to the injected
side. Forty-eight hours after injection, no detectable pERK staining (green)
is observed in LGN neurons with red beads inside the cytoplasm (E,
n=7). (F) Unilateral cortical injections of beads-U0126 do not affect
pERK staining in the dLGN contralateral to the injected cortex; indeed, no red
beads are observed in this side of the brain (n=7). (G,H) Injections
of beads-U0126 in the left side of visual cortex do not affect pERK expression
in the eye. Coronal sections from the retina of the contralateral eye, which
sends the major afferents to the LGN, are shown. pERK immunostaining in the
RGCs of treated animals (G, n=7) is not different from that of
controls (H, n=3). Scale bars: 10 µm for A,E,F; 100 µm for C;
25 µm for D; 20 µm for G,H.
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