|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. lacI-HP1 association silences heat shock inducible expression of
hsp26 and hsp70 reporter genes. (A) The
lac-hsp26-hsp70 reporter contains 256 lac operator sites
positioned 1.9 and 3.7 kb upstream from the transcriptional start site of the
heat shock inducible promoters hsp26 and hsp70,
respectively. The hsp26 gene is fused to a barley cDNA fragment as a
unique sequence tag and hsp70 is fused to the white reporter
gene as a transformation marker. (B) RNA was isolated from adult flies
carrying either the lac-hsp26-hsp70 reporter transposon alone, the
GFP-lacI expressor and the reporter transposon, or the
lacI-HP1 expressor and the reporter transposon. The flies
were raised with daily heat shock treatments or non-heat shock conditions. RNA
was analyzed for hsp26-tag expression (upper autoradiograph)
and rp49 expression (lower autoradiograph) as a loading control. (C)
Northern analysis of hsp70-white expression (upper autoradiograph)
and rp49 expression (lower radiograph).
|
