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Fig. 1. Vegetal ectoderm expresses prospective neural genes at all DV positions. Lateral views of gastrulae (stage indicated in the bottom left-hand corner) with dorsal towards the right. Genes analysed are indicated on the right of the panels with typeface colour reflecting the colour of the respective expression domains. Vegetal marginal ectoderm is indicated by brackets in A and ventrovegetal ectoderm is indicated by arrowheads in other panels. ntl expression indicates prospective mesoderm (A-H), foxi1 and p63 expression shows prospective epidermis (G,H), which overlaps with the animal domain of bmp2b expression (white arrowheads in G-I). foxi1 expression is initiated earlier in this domain than p63. sox3, sox31 and zic2.2 expression labels all prospective neural tissue (A-F) with the exception, for sox3, of a band of cells in the prospective midbrain and hindbrain (asterisk in D). bmp2b, bmp4 and eve1 are expressed in ventral mesoderm at the blastoderm margin in addition to ventrovegetal ectoderm (purple arrowheads in I-K).





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