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Fig. 3. Prospective neural fate in the vegetal ectoderm is initiated independent of
the organiser and antagonism of Bmp activity. Lateral views of gastrula stage
wild-type (A,F,K), chordin mutant (din-/-)
(B,G,L), bmp2b-injected (C,H,M) and ichabod mutant
(ich-/-) (D,E,I,J,N,O) embryos with dorsal towards the
right (when discernable). Genes analysed by in situ hybridisation are
indicated to the left of each row. In E, J and O, embryos were double stained
with a probe for ntl, which marks marginal mesoderm cells. The gap
(black arrowhead) between ntl and p63 expression domains
complementarily expresses sox3 and zic2.2 in
ich-/- embryos. The white arrowheads in A-D indicate the
position at which sox3 is expressed in prospective forebrain neural
tissue in wild-type embryos. Brackets in F-H,K-M indicate the reduction in
prospective anterior neural plate size in din-/-,
bmp2b-injected and ich-/- embryos.
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