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Fig. 1. Defects in Shh signaling in Gli2^-/-;Gli3^xt/xt mutant embryos. (A,B) Widespread and overlapping Gli2 and Gli3 expression in the ventral spinal cord at E10.5. Gli2 expression extends further ventral than Gli3 at this stage (yellow bars mark similar DV position). (C-J) Shh protein expression in the notochord and floorplate (FP). (C,D) Wild-type embryos express Shh in the notochord and FP. (E,F) In Gli2^-/- mutants, FP expression of Shh is selectively lost (arrowhead), while notochord expression is retained. (G,H) In Gli3^xt/xt mutants, Shh expression in the notochord and FP is similar to that in wild type. (I,J) Gli2^-/-;Gli3^xt/xt mutants do not show Shh expression in FP (arrowhead) but notochord expression is similar to Gli2^-/- mutants. (K-N) Ptch1 mRNA expression. Yellow lines indicate wild-type dorsal expression boundary. (K) In wild-type embryos, Ptch1 expression is restricted to the ventral VZ, with higher levels in cells dorsal to the FP (lower arrowhead). Ptch1 is absent in the dorsal VZ (upper arrowhead). (L) In Gli2^-/- mutants, strong ventral Ptch1 expression is not detected (arrowhead), but remaining VZ expression is similar to wild type. (M) In Gli3^xt/xt mutants, Ptch1 expression in the ventral VZ is similar to wild type, but ectopic expression is detected in the dorsal VZ (arrowhead). (N) In Gli2^-/-;Gli3^xt/xt mutants, weak uniform Ptch1 expression is seen (n=3) even in dorsal regions (upper arrowhead). All sections are from E10.5 embryos.

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