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Fig. 2. Complete absence of V3 interneurons in Gli2-/-;Gli3xt/xt mutants. (A,B) In wild-type embryos, Nkx2.2 and Nkx2.9 mark V3 ventral interneuron progenitors that form adjacent to the FP. (C,D) In Gli2-/- mutants, Nkx2.2 expression is greatly reduced, and only a few Nkx2.9 cells could be detected at E10 (C) but not at E10.5 (D). (E,F) In Gli2-/-;Gli3xt/xt mutants, neither protein was detected at any stages or axial level examined.





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