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Fig. 6. Misexpression of dominant activator forms of Gli2 and Gli3 activates ventral and represses dorsal factors. (A-H') Transfections of Gli2 and Gli3 cDNAs encoding N-terminally-truncated forms of the proteins (see schematic) using in-ovo electroporation in chick embryos. (A,B) Neither Gli2 ${Delta}$ N-term nor Gli3 ${Delta}$ N-term induced ectopic Shh expression when transfected at HH stage 12-14. Expansion of epithelium results from Gli2 ${Delta}$ N-term transfections (arrowhead; also see C',E'). (C,D) Gli2 ${Delta}$ N-term induced weak expression of Foxa2/Hnf3ß throughout the spinal cord, while Gli3 ${Delta}$ N-term did not. (E,F) Both Gli2 ${Delta}$ N-term and Gli3 ${Delta}$ N-term induced robust ectopic Nkx2.2 expression throughout the spinal cord. Insets show higher power view of area indicated by arrowhead, excluding the blue channel. (G,H) Gli2 ${Delta}$ N-term, but not Gli3 ${Delta}$ N-term, induced Nkx6.1 expression in the dorsal spinal cord. (I-L) Both Gli2 ${Delta}$ N-term and Gli3 ${Delta}$ N-term suppressed Pax6 and Pax7 expression in the dorsal spinal cord. (M,N) Gli2 ${Delta}$ N-term induced patchy upregulation of Ptch1 and Ptch2 transcription. (O,P) Gli3 ${Delta}$ N-term induced Ptch1 weakly but Ptch2 strongly.

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