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Fig. 5. Rhomboid is required in the CNS to prevent GB midline crossing. (A) The
inga enhancer trap showed weak, but reproducible, reporter gene
expression in GB1. Ventral view of an inga/+ embryo, double stained
for ß-gal and the tracheal lumen (mAb2A12, both in brown). GB1 expression
is shown (arrowheads in A). (B-D) RicinA ablation of most of all GB1 cells (in
a SRF>RicinA embryo) did not affect longitudinal fascicles (stained by
anti-Fas2 in green, B) and glial populations (green in C,D; stained by
anti-Repo and anti-Wrapper respectively, all panels show ventral views). Scale
bar in D: 20 µm. In addition, isolated surviving GBs migrate correctly (B-D
arrows). (E) Rhomboid expression by CNS cells, but not by GB1, rescues the
rho3 GB midline cross phenotype. The table represents the frequency
of midline crosses for the different genotypes. Expression of Rho1 in midline
glia (by slit-Gal4) or in midline cells (by sim-Gal4) halved
the frequency of GB midline crosses in rho3, whereas expression in
all CNS neurons (by elav-Gal4) substantially rescued the
rho3 midline cross phenotype. Expression of the same transgene in GB1
(by SRF-Gal4), did not produce convincing rescue.
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