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Fig. 1. Fgf9-/- XY gonads on the C57BL/6 background do not form
testis cords. Testis cords are visible in wild-type XY gonads by 12.5 dpc
(A,C) and consist of groups of germ cells (red, detected with an antibody
against PECAM) surrounded by a layer of Sertoli cells and enclosed by a basal
lamina (green, detected with an antibody against laminin). PECAM (red) also
labels cells in the vasculature. Fgf9-/- XY gonads on the
C57BL/6 background did not form testis cords (B,D). In wild-type XY gonads,
SF1 (green) is detected in the nuclei of Leydig precursors (ley) outside
testis cords and, at a lower level, in the nuclei of Sertoli cells (s) within
testis cords at 12.5 dpc (examples indicated in E). In
Fgf9-/- XY gonads, a smaller number of SF1-positive cells
were detected, located near the surface of the gonad (F). Laminin (green) and
germ cell (red) localization in Fgf9-/- XX gonads at 12.5
dpc (H) were indistinguishable from Fgf9+/- XX gonads (G).
Scale bars: 50 µm.
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