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Fig. 3. Fgf9-/- XY gonads are smaller than
Fgf9+/- XY littermates by 12.5 dpc. (A) Using PECAM
labeling to define the size of the gonad, it was observed that the width of
Fgf9-/- XY gonads was less than
Fgf9+/- XY gonads and roughly the same as XX gonads at
12.5 dpc. (The width at the gonad's center is indicated by brackets.) (B) The
mean width of gonads from XY individuals (+/+ and +/-) was 277.4±12.5
µm (n=9). Gonads from Fgf9-/- XY littermates
were significantly smaller (Student's t-test P<0.01),
with a mean width of 202.9±14.2 µm (n=6). Gonads from
Fgf9-/- XX individuals had a mean thickness of
187.3±6.8 µm (n=6) and were not significantly smaller than
wild-type XX gonads (193.5±8.5 µm, n=8,
P>0.05). Error bars indicate s.e.m. (C) The mean lengths of gonads
from XY (+/+ and +/-) and Fgf9-/- XY individuals were not
significantly different from each other (1016.3±28.4 µm and
981.5±30.2 µm, respectively, P>0.05). Additionally, the
mean lengths of gonads from XX (+/+ and +/-) and Fgf9-/-
XX individuals were not significantly different from each other
(997.5±13.8 µm and 1025.0±12.7 µm, respectively,
P>0.05).
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