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Fig. 6. FGF binding and induction of proliferation. Gonadal sections were treated
with exogenous human FGF9, which was detected with an antibody specific to the
human protein (green). Human FGF9 bound to cells near the coelomic epithelium
(arrowheads) in XY gonad sections (B), but not XX (A) or XY sections
pretreated with heparatinase (C). Using BrdU labeling, many dividing cells
(red) were observed within cultured XY gonads, concentrated in somatic cells
(labeled with WT1, green) at and near the coelomic surface (D). Fewer dividing
cells were observed in XX gonads (E). Addition of 50 ng/ml FGF9 in the culture
media induced greater numbers of dividing cells in XX gonads. These dividing
cells were concentrated at the surface of the gonad (arrows). Germ cells and
vasculature are blue.
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