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Fig. 8. FGF9 does not induce Sertoli markers but does induce Scc. Nuclear
FGFR2 (green) was specific to Sertoli cells within the cords of wild-type XY
gonads at 12.5 dpc (A). In Fgf9-/- XY gonads, FGFR2 was
found in somatic cells throughout the gonad, but was not found in the nuclei
of any cells (C), in a similar pattern to the Fgf9+/- or
Fgf9-/- XX gonad (B,D). (E-G) FGFR2 is nuclear in cultured
XY gonads (E), but not in XX gonads (F). FGF9 did not induce the nuclear
localization of FGFR2 in cultured XX gonads (G). Germ cells and vasculature
are red. (H) Using in situ hybridization, Scc labels Leydig cells in
the interstitium of XY gonads and Sox9, Amh (Mis in figure)
and Dhh label Sertoli cells within testis cords. These genes were not
expressed in XX gonads at this stage. FGF9 did not induce markers associated
with Sertoli cell differentiation, but did induce Scc expression in
XX gonads. Scale bars: 50 µm in A-G; 250 µm in H.
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