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Fig. 4. Abnormal expression of Xbap in FoxF1 knockdown embryos. (A,B) Whole-mount in-situ hybridization showing Xbap expression on both sides of a CoMo-injected stage 35/36 embryo. A line with a letter in (A) indicates the position of the section in (I). (C,D) Whole-mount in-situ hybridization of Xbap RNA to a FoxF1Mo-injected stage 35/36 embryo. Xbap expression is present on the uninjected side (C) but is absent on the injected side (D). A line with a letter in (C) indicates the position of the section in (J). (E,F) Whole-mount in-situ hybridization showing FoxF1 expression on both sides of a CoMo-injected stage 35/36 embryo. A line with a letter in (E) indicates the position of the section in (K). (G,H) Whole-mount in-situ hybridization of FoxF1 RNA to a FoxF1Mo-injected stage 35/36 embryo. FoxF1 expression is present on both sides of the embryo. A line with a letter in (G) indicates the position of the section in (L). (I) A section through the embryo in (A) shows that Xbap is expressed in the CoMo-injected (shown as right) side as well as on the uninjected (left). (J) A section through the embryo in (C) shows that Xbap is expressed on the uninjected (left) side of a FoxF1Mo-injected embryo but not on the injected (right) side of a tadpole. (K) A section through an embryo hybridized with FoxF1 RNA shows that the lateral plate mesoderm is present on the CoMo-injected (right) side as well as on the uninjected side (left). (L) A section through an embryo hybridized with FoxF1 RNA shows that the lateral plate mesoderm is present on the FoxF1Mo-injected (right) side as well as on the uninjected side (left). Arrows point to the anterior lateral plate mesoderm.

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