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Fig. 5. A lower rate of lateral plate mesoderm proliferation in FoxF1
knockdown embryos. (A-D) Cell proliferation as visualized by BrdU
incorporation (brown nuclear staining). Transverse sections through the
midtrunk of stage-20 embryos injected with CoMo (A) or FoxF1Mo (B)
show drastically reduced cell proliferation in the lateral plate mesoderm of
FoxF1 knockdown embryos. The boxed areas are magnified in (C,D). (C)
Higher magnification of the boxed areas in (A), showing BrdU-positive cells in
the lateral plate mesoderm and in the neuroectoderm (inset) of a CoMo-injected
embryo. (D) Higher magnification of the boxed areas in (B), showing a lack of
BrdU-positive cells in the lateral plate mesoderm but a normal number of
BrdU-positive cells in the neuroectoderm (inset) of a
FoxF1Mo-injected embryo. (E) A column chart showing the numbers of
BrdU-positive nuclei in the lateral plate mesoderm and neuroectoderm of CoMo-
and FoxF1Mo-injected embryos at midtrunk level
(averages±s.e.m.). Nuclei were counted on 10 sections derived from 5
control embryos and 20 sections from 11 FoxF1 knockdown embryos in
two independent experiments. The difference in proliferation rate between
control and knockdown lateral plate mesoderm is statistically significant
(P=5.9 x 10-6 in a two-tailed t-test). LPM: lateral
plate mesoderm. (F,G) TUNEL assay on stage-28 embryos injected with CoMo (F)
and FoxF1Mo (G). Apoptotic cells (blue staining, inset) are mostly
located in the neuroectoderm. No significant differences were observed between
embryos injected with CoMo and FoxF1Mo.
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