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Fig. 7. 5' upstream regulatory sequences of FoxF1 direct gene
expression into the lateral plate mesoderm and are responsive to BMP4
induction. (A) Diagram of a GFP reporter construct used to map the regulatory
sequences of FoxF1 in transgenic embryos. (B) A transgenic
Xenopus tadpole displaying GFP fluorescence in the ventral half of
the body. (C) Same embryo in transmitted light. (D) A cross-section of the
tadpole hybridized with digoxigenin labeled anti-GFP probe showing expression
of the GFP RNA in the lateral plate mesoderm. (E) A cross-section of the
embryo hybridized with digoxigenin labeled anti-GFP probe showing expression
of the GFP RNA on one side of the embryo. (F) Diagram of a LacZ reporter
construct used to map the regulatory sequences of FoxF1 in transgenic
embryos. (G) A transgenic Xenopus embryo displaying LacZ staining in
the ventral half of the body. (H) The pBS-FoxF1-NLS-LacZ reporter construct
does not show any expression of LacZ in cultured animal caps from injected
embryos but shows high levels of expression when co-injected with BMP4 RNA
(I).
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