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Fig. 7. STY protein expression in mutants affecting leaf abaxial-adaxial asymmetry.
(A,B) Consecutive cross sections from the apex of a gram-3 plant
probed with antibody directed against STY (A) and GRAM (B). The arrowhead in A
points to extended STY expression as compared to a wild-type primordium at a
similar developmental stage (arrowhead in
Fig. 5C). Notice lack of signal
in B confirming specificity of the antibody directed against GRAM. (C,D)
Immunolocalisation of STY in cross sections of phan mutant apices
(main shoot in C and axillary shoot in D) from plants grown at low and at
intermediate temperatures. Notice adaxial localisation of the protein in
partially or almost fully abaxialised leaf primordia (white arrowheads). Scale
bars: 100 µm.