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Fig. 6. Interactions between gram, phan and prol mutations.
phan single mutants (A,C) and phan gram double mutants (B,D)
grown at either 20°C or 25°C. gram enhances the abaxialised
phenotype of phan leaves and removes their sensitivity to
temperature. Wild type, gram and phan single mutants produce
a functional embryonic SAM, seen to have a layered structure in an optical
section of a newly germinated seedling (G), that gives rise to a shoot between
the cotyledons (E). phan gram double mutants have abaxialised
cotyledons (F) and fail to form an organised or functional SAM during
embryogenesis (H). The apices of phan mutants grown at 20°C, seen
in transverse section (I) and longitudinal section (J) express GRAM
RNA ectopically in adaxial regions of developing leaves. The needle-like
leaves of phan gram double mutants are radially symmetrical in
transverse section (K), have an abaxialised arrangement of xylem internal to
phloem (L) and express gram RNA ubiquitously (M). (N) prol
gram double mutants also fail to form an embryonic SAM and produce
radially symmetrical leaves (Q) that have the long glandular hairs
characteristic of the wild-type abaxial (P) rather than adaxial (O) midrib;
show abaxialisation of internal cell types (R); ubiquitous expression of
gram RNA (S) and reduced expression of AmPHB RNA (T),
although AmPHB expression remains in the SAM (arrowhead).
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