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Fig. 8. The first monoaminergic neurones in trochophores of Helisoma and
Lymnaea at stage 19 (20%). (A,C-F) Anti-tubulin Immunostaining (red).
(A) Helisoma; anti-5-HT immunolabelling (green). Anterior sensory
neurones (arrows) are located on both sides of the mouth (m), and project to
the pedal ciliary band (pb); pn, cilia in protonephridia (LSM projection,
48x0.6 µm). (B) Lymnaea; glyoxylic acid-induced fluorescence
of catecholamines; conventional epifluorescence. The pair of the first
anterior cells (arrows) is located above the mouth (m). (C) Lymnaea;
anti-tyrosine hydroxylase immunolabelling (green). Anterior neurones (arrows)
make a varicose network underneath the apical ciliary plate (LSM projection,
26x1 µm). (D) Lymnaea; anti-5-HT immunolabelling (green).
High power image of the anterior neurone with a short thick ciliated apical
fibre, thin basal fibre and two short lateral fibres (arrows) emanating from
the soma (LSM projection, 54x0.35 µm). (E) Helisoma;
anti-5-HT immunolabelling (green). The anterior neurone also has a thick
ciliated apical fibre, a thin basal fibre and two short lateral fibres
(arrows) (LSM projection, 43x0.3 µm). (F) Helisoma;
anti-5-HT immunolabelling (green). Varicose network made by the fibres of the
anterior neurones underneath the pedal ciliary band (pb) (LSM projection,
26x0.4 µm). Scale bars: 20 µm in A,B; 10 µm in C-F.
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