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Fig. 2. fgf3 mutant embryos display defects in otic vesicles, ventral head
skeleton and pharyngeal endoderm. Probes used in whole-mount in-situ
hybridizations are indicated in the lower right corners, ages of embryos in
the upper right corners. Left panels (A,C,E,G) show wild-type siblings (wt),
right panels (B,D,F,H) fgf3 mutants (lia). Embryos were
genotyped after photography. (A-F) Lateral views. (G,H) Dorsal views on heads.
`k' in C marks krox20 in rhombomeres 3 and 5. Arrows and numbers in
C,D mark neural crest streams to the corresponding pharyngeal arches (I,
mandibular; II, hyoid; III, IV, V, gill arches). Arrows in E mark pharyngeal
pouches of gill arches. (G,H) Alcian Blue staining of craniofacial cartilage;
arrows in G indicate ceratobranchials of the gill arches, arrow in H remaining
part of first ceratobranchial of fgf3 mutant. Arrowheads mark
otoliths, which are fused in the mutant. bb, basibranchial; cb,
ceratobranchials; ch, ceratohyal; i, isthmus; mb, mandibulare; nc, neural
crest; ppe, pharyngeal pouch endoderm; pt, pallial (dorsal) telencephalon; os,
optic stalk; spt, subpallial telencephalon; vt, ventral thalamus; pvh,
posterior-ventral hypothalamus (infundibulum; presumptive
neurohypophysis).
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