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Fig. 5. Fgf3 signaling from the diencephalon, but not the telencephalon, is
sufficient for adenohypophyseal development. (A,C-H) Embryos at 32 hpf,
lateral views (A,C,F,G), or frontal views (D,F,H), on head regions. (A) Embryo
stained for fgf3 transcripts. Note the fissure of the eye vesicle as
a reference point for the anterior border of the infundibular fgf3
expression domain. (B) Cartoon showing the transplantation sites at shield
stages. Consistent with results obtained in fate-mapping experiments
(Woo and Fraser, 1995;
Varga et al., 1999;
Mathieu et al., 2002),
telencephalic chimeras as shown in G,H were obtained by transplanting cells
from/to the animal pole, diencephalic chimeras as shown in C-F by
transplanting cells from/to dorsal regions anterior/animal of the shield.
Cells of the adenohypophysis (ad) are supposed to derive from region indicated
by arrowhead. (C-H) Chimeras, in-situ hybridized for lim3 transcripts
in blue, and with transplanted wild-type cells in brown. Out-of-focus
bilateral lim3 domains in D,F,H represent hindbrain motoneurons
(Glasgow et al., 1997). (C,D)
Wild-type recipients with wild-type donor cells in the diencephalon,
displaying normal adenohypophyseal lim3 expression (indicated by
arrows). (E,F) fgf3 mutant recipients with infundibular wild-type
cells (indicated by arrowheads) and adjacent rescued adenohypophyseal
lim3 expression (indicated by arrows). Inset in E shows second
rescued embryo. (G,H) fgf3 mutant recipient with many wild-type cells
in the telencephalon, still lacking adenohypophyseal lim3
expression.
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