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Fig. 6. Fgf signaling governing adenohypophysis development is required during midsegmentation stages. All panels show embryos after lim3 in-situ hybridization. Adenohypophyseal lim3 staining in (A-C,E,G) is indicated by arrows. (A-D) Bead implantations, performed at 18 hpf. Panels show lateral views on heads of embryos at 26 hpf. Embryos were genotyped after photography. (A) Implant of Fgf3 bead into wild-type sibling embryo. (B,C) Implant of Fgf3 beads into mutant embryos, leading to partial rescue of adenohypophyseal lim3 expression; note bead position far from the telencephalon in (B). (D) Implant of BSA bead into mutant embryo. (E-H) SU5402 treatments. (E,F) Lateral views, (G,H) frontal views on heads of embryos at 34 hpf. Incubation of embryo in 20 µM SU5402 from 18-22 hpf leads to complete loss of adenohypophyseal lim3 expression, while control embryo treated with DMSO is unaffected.





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