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Fig. 5. Time-lapse observation of Rohon-Beard neurons that were separately labeled
with Kaede in the embryos with a reduced level of PlexinA4 expression or with
ubiquitous overexpression of Slit2. Stacked confocal images of a single
Rohon-Beard neuron expressing Kaede transiently under control of the primary
sensory neuron-specific enhancer (SS) of the zebrafish isl1 gene
(A-F). In comparison with normal embryo (A,D,A',D'), the number of
branching points of peripheral axons was reduced in the embryo injected with
AMO against plexinA4 (B,E,B',E'). However, the number of
branching points increased in the Slit2-overexpressing embryo
(C,F,C',F'). (A-F) Confocal images; lateral view; anterior
leftwards; dorsal towards the top. (A-C) 23 hpf embryos. (D-F) The same
embryos as shown in A-C at 27 hpf. (A'-F') Schematic drawings of
A-F in which cell bodies and branching points of the peripheral axons were
indicated by black circles and red dots, respectively. Scale bar: 10
µm.
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