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Fig. 1. (A) Epifluorescence photomicrograph of layer 2 and III pyramidal neurons
showing stable labeling with GFP 24 hours after biolistic transfection with a
GFP expression plasmid. (B) Z stack projection of a representative layer 2
pyramidal neuron after 48 hours in culture constructed from 10 confocal images
of the neuron obtained at 10 µm intervals through the dendritic arbor.
Scale bars: 50 µm. (C) RT-PCR analysis of HGF and MET expression in freshly
prepared cortical slices and in cortical slices that had been cultured for 2
days. The PCR products for HGF, MET and GAPDH are indicated. No amplification
products are observed in control reactions in which the reverse transcription
(RT) step was omitted.
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