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Fig. 3. Quantitative analysis of the effect of different treatments on the number
of primary dendrites. Cortical slices were grown under control conditions
(Ctr), or in medium supplemented with 200 ng/ml HGF (HGF), 1 µg/ml anti-HGF
(aHGF), 200 ng/ml HGF plus 1 µg/ml anti-HGF pre-incubated for 1 hour before
addition to the cultures (HGF + a1), 200 ng/ml HGF plus 3 µg/ml anti-HGF
pre-incubated for 1 hour before addition to the cultures (HGF + a2), 200 ng/ml
BDNF (BDNF) or HGF + BDNF. The bar charts illustrate the total number of
primary dendrites. *P<0.05. Between 50 and 60
individual neurons were analysed under each condition.