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Fig. 10. Expression of KNU:GUS constructions in wild-type and knu genetic backgrounds. The three GUS constructions shown in Fig. 9 were introduced into Arabidopsis wild-type (A-M), and knu mutant backgrounds (N-P). Patterns of GUS activity were examined in whole-mounts or thin sections (D-F,M). (A-C) Inflorescences. (D-G) Representative expression in developing flower buds. (H) Detail of an anther; I-L show details of developing ovules. Images B,D-I,K were taken from primary transgenics, and the remainder from homozygous plants. The introduced construct is named in the top right-hand corner of each panel, and the developmental stage in the bottom-left corner (where relevant). Scale bars: 25 µm in D-F,H-L,M,N; 50 µm in O,P; 100 µm in G; 1 mm in A-C, inset. a, anther; ecp, ectopic carpel primordium; ep, ectopic primordium; esp, ectopic stamen primordia; ems, embryo sac; gy, gynoecium or gynoecial primordium; ii, inner integument of ovule; mmc, megaspore mother cell; met, megaspore tetrad; mit, microspore tetrad; op, ovule primordium; ov, ovules; pe, petal; se, sepal; st, stamen or stamen primordium.





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