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Fig. 7. RT-PCR demonstrates that KNUCKLES is transcribed at low levels in
green flower buds but not in anthesis-stage flowers. Ethidium bromide-stained
3% TAE agarose gel. First row, 869 bp fragment of ß-tubulin
transcript cDNA amplified after 30 cycles. Second row, 332 bp KNU
transcript cDNA fragment amplified after 40 cycles, loaded 2:1 relative to
volumetric proportion of 50 µL ß-tubulin reaction
(ßtub). Positive control amplifications from plasmid templates
are denoted (+). Tissue sampled included: floral apices, including green buds
up to floral stage 11 (FA), stage 12 buds with petals showing (PS), and open
flowers at stage 13 with dehisced anthers (AN), in addition to floral apices
from the knuckles mutant (knu).
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