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Fig. 9. Complementation and GUS reporter constructs and their effects in transgenic
Arabidopsis. (A) Complementation constructs. Plasmids listed far
left. Binary T-DNA parent plasmids shown in parentheses. Centre, inserts are
depicted as coloured bars. KNU coding sequences are shown in red, and
flanking genomic sequences are shown in yellow. Far right,
hygromycin-resistant knu transgenics were scored as being fully or
partially complemented or uncomplemented based on restoration of fertility and
morphological criteria. (B) GUS reporter constructs. The uidA gene is
shown as a blue bar. Far right, cartoons representing stage 6 and 9 flower
buds, early ovules, and leaf and stem are shaded blue to approximate patterns
of GUS staining at the stages depicted in multiple independent lines
containing each construct. Scale in kilobases (Kb). H, HindIII; N,
NcoI; P, PstI; X, XbaI; Xm,
methylation-sensitive XbaI site.
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